মঙ্গলবার, ২৫ জুন, ২০১৩

Jon Gosselin: I live in the woods now

Celebs

12 hours ago

IMAGE: Jon Gosselin

Michael Buckner / Getty Images file

Jon Gosselin in 2012.

Once Jon Gosselin lived in a large house in Pennsylvania and his life unrolled on television in front of millions. Now, the dad of eight lives "in the woods," and says he doesn't even have an address.

Gosselin didn't clarify exactly where or how he lives, but he confirmed to VH1's "The Gossip Table" that he's taken to a more private life after living in an apartment where paparazzi and others "figured out where I was."

Gosselin, then-wife Kate, and their twins and sextuplets starred on "Jon and Kate Plus 8" for five seasons before divorcing. The show continued as "Kate Plus 8" despite Jon Gosselin suing to prevent filming of his children.

Gosselin was asked if Kim Kardashian and Kanye West should allow their newborn daughter, North, to appear on the reality show "Keeping Up With the Kardashians," and unsurprisingly, he didn't think so.

"I wanted to raise my kids off television, so I changed my mind," he said. "So I would definitely not film with my newborn child."

Gosselin was also asked if he still wore Ed Hardy clothing, the brand he favored at the height of his tabloid fame in 2009. He said no, adding "I gave all my (Hardy clothing) to my mother." Tattoo artist Hardy recently told the New York Post that an association with Gosselin "tanked" his clothing brand.

He also said he'd be interested in appearing on "Dancing With the Stars," as his ex-wife Kate Gosselin famously did in 2010. She was the fourth celebrity eliminated on the show's tenth season, and partner Tony Dovolani later joked he needed "a lot of therapy" after partnering with her.

"I feel like I could probably get further than her," Jon Gosselin said.

Source: http://www.today.com/entertainment/jon-gosselin-i-live-woods-now-6C10433570

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Light turnout predicted as Mass. picks US senator

This panel of May 2013 file photos shows Republican Gabriel Gomez, left, and Democrat U.S. Rep. Ed Markey, right, candidates for U.S. Senate in the June 25, 2013 special election, being held to fill the seat vacated when John Kerry was appointed as secretary of state. (AP Photos/File)

This panel of May 2013 file photos shows Republican Gabriel Gomez, left, and Democrat U.S. Rep. Ed Markey, right, candidates for U.S. Senate in the June 25, 2013 special election, being held to fill the seat vacated when John Kerry was appointed as secretary of state. (AP Photos/File)

Massachusetts Senate Democratic candidate Ed Markey, left, meets and greets grassroots volunteers and supporters at the Pickle Barrel Restaurant & Deli, in Worcester, Mass., Monday, June 24, 2013. Markey and Republican Gabriel Gomez made appeals to voters Monday in the final hours before Massachusetts' special election for the U.S. Senate, where turnout is expected to be light, a contrast to the high-profile special election in the state three years ago. (AP Photo/Worcester Telegram & Gazette, John Ferrarone)

Gabriel Gomez, Republican candidate for U.S. Senate in the Massachusetts open seat special election, greets supporters, Monday, June 24, 2013, at the Four Square restaurant in Braintree, Mass. Gomez faces Democrat Rep. Ed Markey in Tuesday's election. (AP Photo/Josh Reynolds)

Massachusetts Senate Democratic candidate Ed Markey, right, meets and greets grassroots volunteers and supporters at the Pickle Barrel Restaurant & Deli, in Worcester, Mass., Monday, June 24, 2013. Markey and Republican Gabriel Gomez made appeals to voters Monday in the final hours before Massachusetts' special election for the U.S. Senate, where turnout is expected to be light, a contrast to the high-profile special election in the state three years ago. (AP Photo/Worcester Telegram & Gazette, John Ferrarone)

Gabriel Gomez, Republican candidate for U.S. Senate in the Massachusetts open seat special election, greets supporters, Monday, June 24, 2013, at the Four Square restaurant in Braintree, Mass. Gomez faces Democrat Rep. Ed Markey in Tuesday's election. (AP Photo/Josh Reynolds)

(AP) ? Democratic U.S. Rep. Edward Markey and Republican Gabriel Gomez both expressed confidence in the messages they delivered to voters during their campaigns to succeed John Kerry in the U.S. Senate, a race where relatively few people were expected to vote in Tuesday's special election.

Both candidates made a series of stops in the campaigns' final hours, culminating with election eve rallies Monday night, while their staffers cranked up their all-important ground games designed to get as many of their voters to the polls as possible.

On Tuesday, both men themselves voted and reflected briefly on the shortened campaign season. The Senate seat opened when Kerry resigned to become U.S. secretary of state.

Markey, 66, voted with his wife in his hometown of Malden. He said the length of the campaign didn't prevent him from repeatedly crisscrossing the state and letting voters know what he stands for. He has said his campaign called or rang the doorbells of 3 million prospective voters in the past several days.

"I have delivered a message on gun safety, on a woman's right to choose, on creating more jobs and I think that message has been delivered and I feel very good about today," he said. "And tonight we gonna have a very good night."

Markey has led in the polls but said Monday that he's taking nothing for granted.

"There is no overconfidence in this entire operation," Markey told reporters after an evening rally in Malden.

Gomez, 47, a political newcomer who worked for a Boston-based private equity firm before jumping into the race, voted in Cohasset, where he lives with his wife and four children. He said he was humbled and proud of the opportunity to vote for himself, saying the election was about choosing the future over the past and what he called Markey's failure to take on the important issues, despite 37 years in office.

"Where I come from, that is mission incomplete," said the former Navy SEAL, adding he was asking for just 17 months, the remainder of Kerry's term.

"Give me a chance to go down there for 17 months and accomplish the mission, which I've done all my life," Gomez said.

In Cambridge, Lori Berenson, 51, said she voted for Markey, mainly because she was skeptical of one of Gomez's main campaign pitches: his request for just 17 months in office.

"He thinks in 17 months he's going to accomplish what Markey hasn't done in 37 years?" she said.

David Wanders, a 43-year-old union member from Stoughton, said he voted for Gomez largely because Markey has been in Washington too long already.

"He's a lifer," said Wanders, an independent. "I don't think he lives here. He lives in Washington."

Wanders, who voted for President Barack Obama in the last election, said nothing in particular attracted him to Gomez.

"What's 17 months going to hurt?" he asked. "If we don't like him, we can get rid of him."

Massachusetts state Secretary William Galvin said Monday that he expected a lackluster turnout, with no more than 1.6 million of the state's 4.3 million registered voters to cast ballots in the special election, well below the 2.2 million who voted in a 2010 special election, won by Republican Scott Brown, to succeed the late Sen. Edward Kennedy.

As of 3 p.m. in Boston, 48,868 voters had cast ballots, or about 12.5 percent of the city's eligible voters. That's far less than the 81,882 Boston voters who had cast ballots at the same point in the 2010 special U.S. Senate election.

Statewide turnout was not immediately available.

Markey has held a fundraising advantage throughout the campaign, having spent more $8.6 million on the race through the end of the last reporting period June 5, compared with $2.3 million by Gomez, according to Federal Election Commission records.

Also on the ballot is Richard Heos, who is affiliated with the Twelve Visions Party.

Polls are open until 8 p.m.

___

Associated Press writer Steve Peoples contributed to this report.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/89ae8247abe8493fae24405546e9a1aa/Article_2013-06-25-Massachusetts%20Senate/id-4a6cc5ee8c3a48e98cc7accef211aa34

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Foursquare for Android and iOS now lets you check friends in, with permission

Foursquare for Android and iOS now lets you check friends in, thankfully with permission

If you regularly hang out with Foursquare aficionados, you've likely seen conversations grind to a halt as everyone dutifully checks in at the same restaurant. A fresh update to Foursquare's Android and iOS apps could get those friends talking again by letting one of them check in the rest. Whoever arrives first just has to tag their contacts, who'll be counted as if they'd gone through the check-in themselves. Thankfully, Foursquare tries to eliminate the privacy disasters that could stem from its new feature -- the app won't check anyone in without permission, and users can delete unwanted check-ins on the spot. Those comfortable with Foursquare's safeguards can grab the new app through the source links.

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Source: http://www.engadget.com/2013/06/25/foursquare-for-android-and-ios-now-lets-you-check-friends-in/?utm_medium=feed&utm_source=Feed_Classic&utm_campaign=Engadget

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Immigration bill's step toward final passage

Tennessee Republican Sen. Bob Corker (l) and North Dakota Sen. John Hoeven (r). (Win McNamee/Getty Images)

After just a few hours of floor speeches Monday afternoon, the Senate voted 67-27 to end debate on an amendment to the immigration bill that would increase border seucirty funding, taking another step toward the legislation's final passage.

Fifteen Republicans joined Democrats in support of a motion to end debate on the amendment, which required 60 votes to proceed.

As part of an agreement between Republican Sen. Bob Corker of Tennessee and Democratic Sen. John Hoeven of North Dakota, the amendment is intended to ease concerns of skeptical lawmakers who are calling for tougher border enforcement as part of the bill.

The Corker-Hoeven amendment retains much of the language of the original bill proposed by a bipartisan group of eight senators earlier this year, but adds 119 new pages that would strengthen security measures by nearly doubling the amount of security agents along the nation's borders. The bill would also mandate the construction of a fence stretching "no less than" 700 miles along the U.S. border with Mexico and provide funding for aerial surveillance of the area. The federal government will be required to meet a series of security benchmarks before immigrants living in the country illegally would be allowed to obtain permanent legal status.

?The American people want a strong, comprehensive immigration reform plan, but we need to get it right,? Hoeven said in a statement last week. ?That means first and foremost securing the southern border before we address other meaningful reforms to our immigration policy. They want to know that ten years from now, we won?t find ourselves in this same position, having to address the same problem.?

Lawmakers rejected a similar (and less costly) amendment to the bill proposed by Texas Republican Sen. John Cornyn last week by tabling the measure, so supporters of the new amendment hope it will serve as a vessel to entice more Republicans to sign on to the bill.

Before the vote, many Republicans stressed that they did not have enough time to read and debate the amendment, which was introduced late Friday afternoon after many lawmakers had already left Washington.

"It continues to be my hope that we can do something lasting and important about our broken immigration system. Living up to our commitments to our constituents, particularly with respect to border security, has long been of critical importance for me," Senate Minority Leader Mitch McConnell, who voted against the motion to proceed on the amendment, said in a statement Monday. "Sadly, I?m not convinced that this amendment solves that problem, and I see no good reason why we would need to vote on it so hastily."

Speaking on the Senate floor Monday before the vote, Corker responded to critics, saying that the new measures in the bill as part of the amendment would take less than an hour to read over the weekend.

"It's not as if something has been dropped on people that is from outer space," Corker said. "This is 119 pages, it's easy to read. All of us could read it in a short amount of time."

The co-authors of the original immigration bill, including Democratic Sen. Chuck Schumer of New York and Republican Sen. Lindsey Graham of South Carolina, have spoken optimistically about securing as many as 70 votes for the final bill in the Senate, the number they think the bill needs to show the effort has wide bipartisan support. The bill is likely to get the support from 60 members needed to overcome a filibuster, but getting 70 would put pressure on the House?a chamber with a higher concentration of conservative lawmakers?to act.

?We?re very, very close to getting 70 votes," Graham said during a weekend interview on ?Fox News Sunday.?

Source: http://news.yahoo.com/blogs/ticket/immigration-bill-faces-another-major-hurdle-senate-monday-151728618.html

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সোমবার, ২৪ জুন, ২০১৩

Successful Experts Talk about Their Personal Injury Legal Advice ...

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Source: http://progvariada.net/sin-categoria/successful-experts-talk-about-their-personal-injury-legal-advice-with-you/

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Israel approves export of 40 percent of its gas

JERUSALEM (AP) ? The Israeli Cabinet on Sunday approved exporting 40 percent of Israel's newfound natural gas reserves, keeping a larger amount for local consumption than originally expected.

Prime Minister Benjamin Netanyahu told his Cabinet the decision struck a balance between domestic needs and the concerns of the exploration companies that will drill for gas underneath the Mediterranean Sea.

"It ensures the needs of the citizens of the state of Israel, both by filling the state coffers with considerable funds from exports and by supplying the local market with cheap energy," Netanyahu said.

Last year, an advisory panel proposed exporting just over half of the country's gas, sparking protests by Israelis who said the country should keep most of its reserves to reduce energy prices at home.

Israel began pumping gas from the large Tamar field off its coast earlier this year. It is expected to begin exporting when a second, larger field goes online in 2016.

The consortium that has developed the fields, led by U.S. company Noble Energy, did not immediately comment on Sunday's decision. In the past, it has said it would have preferred the larger export levels.

Hebrew University professor Eytan Sheshinski, an expert on energy policy, said that despite the export numbers, he expected the energy companies to be satisfied with Sunday's decision.

"When the dust settles, they can live with this decision, and I think it didn't cross their red line," said Sheshinski, who headed a committee that gave policy recommendations to the Israeli government in 2010 on taxing natural gas exports.

Sunday's decision reserved 540 billion cubic meters of natural gas for the domestic market. Netanyahu said that amount would supply Israelis with natural gas for at least 25 years, a figure that Sheshinski said was largely accurate.

Earlier in the month, Environment Minister Amir Peretz said he wanted at least 600 billion cubic meters set aside for local use.

Last week, Netanyahu said that Israel seeks to earn $60 billion over the next two decades from the exports.

Source: http://news.yahoo.com/israel-approves-export-40-percent-gas-155022171.html

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Sharapova sends verbal shot Serena Williams' way

Russian tennis player, Maria Sharapova, left, watches Grigor Dimitrov of Bulgaria plays Dudi Sela of Israel during their Queen's Club grass court championships first round tennis match in London, Monday, June 10, 2013. (AP Photo/Sang Tan)

Russian tennis player, Maria Sharapova, left, watches Grigor Dimitrov of Bulgaria plays Dudi Sela of Israel during their Queen's Club grass court championships first round tennis match in London, Monday, June 10, 2013. (AP Photo/Sang Tan)

Serena Williams, of the U.S, reacts as she defeats Russia's Maria Sharapova during the Women's final match of the French Open tennis tournament at the Roland Garros stadium Saturday, June 8, 2013 in Paris. Williams won 6-4, 6-4. (AP Photo/Christophe Ena)

LONDON (AP) ? Maria Sharapova took quite a shot at Serena Williams ? and it was nowhere near a tennis court.

At her pre-Wimbledon news conference Saturday, Sharapova was asked about a recent Rolling Stone article where the author surmised that critical comments directed at an unnamed player by Williams were referring to Sharapova.

"At the end of the day, we have a tremendous amount of respect for what we do on the court. I just think she should be talking about her accomplishments, her achievements, rather than everything else that's just getting attention and controversy," Sharapova said.

"If she wants to talk about something personal, maybe she should talk about her relationship and her boyfriend that was married and is getting a divorce and has kids," Sharapova continued. "Talk about other things, but not draw attention to other things. She has so much in her life, many positives, and I think that's what it should be about."

Williams has been linked to coach Patrick Mouratoglou, but neither has confirmed their relationship extends beyond the court. When Mouratoglou was asked about the topic at the French Open this month, he smiled and replied: "Sorry. I don't understand the question."

According to the Rolling Stone story, posted online Tuesday, Williams spoke about what the reporter described as "a top-five player who is now in love."

Williams is quoted as saying: "She begins every interview with 'I'm so happy. I'm so lucky' ? it's so boring. She's still not going to be invited to the cool parties. And, hey, if she wants to be with the guy with a black heart, go for it."

That is followed by these words in parentheses from the author of the piece, Stephen Rodrick: "An educated guess is she's talking about Sharapova, who is now dating Grigor Dimitrov, one of Serena's rumored exes."

Sharapova beat Williams in the 2004 Wimbledon final. But Williams has won their past 13 matches in a row, including in the French Open final two weeks ago.

At Wimbledon, where play begins Monday, Williams is the defending champion and seeded No. 1. Sharapova is seeded No. 3. They only could face each other in the final.

Williams is scheduled to hold a pre-tournament news conference at Wimbledon on Sunday.

The Rolling Stone article, which was about 4,000 words, drew widespread attention mostly for a one-paragraph reference to the Steubenville rape case. Williams is quoted as saying the teenage victim "shouldn't have put herself in that position."

Two players from the Steubenville, Ohio, high school football team were convicted in March of raping a drunken 16-year-old girl; one of the boys was ordered to serve an additional year for photographing the girl naked. The case gained widespread attention in part because of the callousness with which other students used social media to gossip about it.

A day after the story was posted, Williams issued a statement in which she said she was "reaching out to the girl's family to let her know that I am deeply sorry for what was written."

Williams' statement continued: "What was written ? what I supposedly said ? is insensitive and hurtful, and I by no means would say or insinuate that she was at all to blame."

Said Sharapova on Saturday: "I was definitely sad to hear what she had to say about the whole case."

___

Follow Howard Fendrich on Twitter at http://twitter.com/HowardFendrich

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/347875155d53465d95cec892aeb06419/Article_2013-06-22-Wimbledon-Sharapova-SWilliams/id-b939b5543167426ba74a86bb87d3bc68

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Police again search home of Patriots' Hernandez

NORTH ATTLEBORO, Mass. (AP) ? State police officers and dogs are searching the home of New England Patriots tight end Aaron Hernandez as they investigate the killing of a semi-pro football player whose body was found nearby.

Saturday's search of Hernandez's sprawling home in North Attleboro involved several officers.

Police have previously searched in and around the home as they try to figure out who killed Odin Lloyd, whose body was found about a mile from Hernandez's home.

Lloyd's family says the two men were friends and together the night he died. Authorities have ruled Lloyd's death a homicide.

A spokeswoman for the Bristol District Attorney's office declined to comment on the investigation Saturday.

An attorney for Hernandez has said he would not comment on the searches.

Source: http://news.yahoo.com/police-again-search-home-patriots-hernandez-205013835.html

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শনিবার, ২২ জুন, ২০১৩

Home-Cooked Challenge: Kids in the Kitchen - NYTimes.com

[unable to retrieve full-text content]How much can children do in the kitchen, and how can parents help them do more? ... ?People told me they had no idea that you could involve the children,? she said, ?but for most families it's not an option not to! ... and if breakfasts, lunches and snacks are going to involve something more complex than the toaster oven and a Pop Tart (I'm a little appalled by how Pop Tart dependent we've become), they're going to have to step up ? and maybe more important, I have ...

Source: http://parenting.blogs.nytimes.com/2013/06/21/home-cooked-challenge-children-in-the-kitchen/

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Unemployment fell in half of US states in May

In this Thursday, May 30, 2013, photo, job seekers line up to talk to recruiters during a job fair held in Atlanta. The government reports on state unemployment rates for May, on Friday, June 21, 2013. (AP Photo/John Amis)

In this Thursday, May 30, 2013, photo, job seekers line up to talk to recruiters during a job fair held in Atlanta. The government reports on state unemployment rates for May, on Friday, June 21, 2013. (AP Photo/John Amis)

(AP) ? Unemployment rates fell in half of U.S. states last month, led by drops in California, West Virginia, New York and Hawaii.

The Labor Department said Friday that unemployment rates rose in 17 states and were unchanged in eight.

Hiring has been steady nationwide, leading to a better job market in many areas of the country. Employers added jobs in 33 states last month. The biggest gains were in Ohio, Texas and Michigan.

The unemployment rate dipped in the Northeast to 7.5 percent from 7.6 percent, and fell in the West to 7.8 percent from 8 percent. It was flat in the Midwest at 7.2 percent and edged up in the South to 7.2 percent from 7.1 percent.

California and West Virginia had the largest declines in unemployment among all states. In California, the rate dropped to 8.6 percent from 9 percent in April. West Virginia's rate fell to 6.2 percent from 6.6 percent.

Both states reported job gains.

California has also seen the largest drop in unemployment among the states in the past 12 months. Its rate fell 2.1 percentage points from May 2012. Nevada's decline of 2 percent was the second largest.

New York and Hawaii also had significant declines in May from April. New York's unemployment rate dropped to 7.6 percent from 7.8 percent, while Hawaii's fell to 4.7 percent from 4.9 percent.

North Dakota had the nation's lowest unemployment rate at 3.2 percent.

Nevada had the highest at 9.5 percent. It was followed by Illinois and Mississippi, each at 9.1 percent.

Nationally, the economy added 175,000 jobs in May, nearly matching the average monthly gain for the past year. The unemployment rate ticked up to 7.6 percent from 7.5 percent, but for a good reason: More Americans were confident they could find work and began searching for a job.

The Federal Reserve on Wednesday offered a brighter outlook for the job market and economy. And Chairman Ben Bernanke said the Fed is likely to slow its bond-buying program later this year and end it next year if the economy continues to strengthen.

Fed officials now expect the unemployment rate to fall as low 7.2 percent this year and between 6.5 percent and 6.8 percent by the end of 2014.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/f70471f764144b2fab526d39972d37b3/Article_2013-06-21-State%20Unemployment/id-f24c51b329e84309a600ebc7bfc0682f

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Gandolfini remembered as fighter for vets

Celebs

2 hours ago

James Gandolfini, who rose to fame as Tony Soprano on ?The Sopranos,? loved playing the complicated mobster. It is likely the role he?ll be most remembered for after his unexpected passing on June 19.

But as much fame and recognition as the role brought him, he was at heart a blue-collared guy who seemed just as proud of his HBO projects that involved bringing attention to the plight of veterans: 2007's "Alive Day Memories: Home From Iraq" and 2010's "Wartorn: 1861-2010."

Image: "Wartorn 1861-2010"

HBO

James Gandolfini speaks to Gen. Peter W. Chiarelli in "Wartorn 1861-2010."

?Alive Day Memories,? which he both hosted and executive produced, took a look at wounded soldiers and the physical and emotional cost of the Iraq War.

?Coming right on the heels of ?The Sopranos? controversial season finale, this showed such a different and impassioned side of James Gandolfini: so soft-spoken and careful in his sensitive interviews with grievously wounded veterans. If anyone ever doubted that the actor was a world removed from the conflicted brute he played so brilliantly on TV, this documentary reinforced the ?gentle giant? side of his personality,? Matt Roush, senior TV critic for TV Guide, told NBC News. ?He obviously admired and respected these men and women and felt it a privilege to let them tell their stories through him. Hard to imagine a better use of one's celebrity and clout than getting the home network (to whom he stayed remarkably loyal, and vice versa) to expose this project to a wide audience.?

Gandolfini, who was never a fan of answering questions from throngs of reporters, set aside his own feelings and attended the Television Critics Association?s 2007 summer press tour to promote the project.

?I went to Iraq because I was playing this tough guy on TV, and I guess I wanted to go meet a few real ones. I was angry about the lack of attention that was being paid,? Gandolfini told reporters. ?I thought it was the least I could do.?

Gandolfini made the trip to Iraq two years before filming the documentary. Of the trip, he said, ?I met a lot of people and I met the soldiers. And then I came home and it was like, there?s nothing here (on TV about the impact of the war on soldiers). What?s going on? (HBO) came to me and I said, ?Yeah. Whatever I can do.??

Sheila Nevins, the president of HBO Documentary Films, explained to reporters at the press tour that the network had wanted to do a documentary about veterans of the Iraq War. ?We knew this was not an easy thing to watch, and it was not something necessarily that we could get people to watch,? she added.

But she knew that with Gandolfini on board, viewers would tune in. ?We spent a day with him at Walter Reed, watching him go from bed to bed and mother to mother,? she said. ?I knew I actually had a way possibly to make people watch these young men and women who were coming home.?

During the panel, the actor made sure the focus stayed on the wounded warriors who were there sharing the stage with him, deflecting any questions about himself and redirecting back to the subjects of the documentary.

?It?s not about me,? he told reporters when asked about how the project impacted him. ?I?m not trying to be antagonistic in any way, but I?d like the questions directed towards other things besides how it changed me, you know what I?m saying? Let?s have a different question.?

The veterans involved in the documentary praised him for being a good listener and setting aside his own star status to put their stories front and center. Not only that, they said the star was anything but a celebrity when he worked with them.

?You weren?t talking to Tony Soprano,? veteran Jay Wilkerson, who is featured in the documentary, said of speaking with the actor for the film. ?You were talking to this man who cared about us and our stories. He listened, really listened to what we had to say.?

?He made me feel like I was open to say anything and everything I wanted to say, and I had no boundaries,? the vet also said. ?And that?s what I was never able to do in Iraq. I was always told not to do that. He made it possible. And so I opened my mouth and spoke, and it was exactly what happened, word for word.?

Veteran Jonathan Bartlett, who lost his legs in the war, said the actor seemed a bit intimidating when they first met, but after they started talking, Gandolfini listened.

"There?s a lot of people, when you try to talk about this? stuff, it?s not something they want to hear about," Barlett told reporters at press tour. "We?re? talking about the way I died, talking about the way my legs? were torn off, talking about the way I almost lost my eye, ?talking about the way my dreams were shattered, and the man ?I thought I was is still living in me and he?s blown to ?crap. That?s hard to articulate. We sat, we got? comfortable, and we just let it all out, and that?s very,? very nice."

After the presentation, Gandolfini kept his own dislike for talking to member of the press at bay for the greater good of promoting the project. At an HBO party that hot July night, reporters instructed by their editors to get something from Gandolfini about the controversial ?Sopranos? series final that aired the month before tried breaching the wall with zero success. Gandolfini was tossing back appletinis and talked only about the veterans surrounding him.

When one reporter he was familiar with slipped in a ?Sopranos? question during a more indepth interview, instead of getting angry, he broke into a smile. Then he laughed and put his arms around the writer and whispered in her ear.

?That was a really good one, and almost makes me want to answer, but all this isn?t about me. It?s about them,? said Gandolfini.

Everyone thought the reporter had gotten a scoop. And she did: confirmation that Gandolfini cared more about the people in the documentary than in exploiting the opportunity to get more publicity out of his higher profile role.

A rare thing indeed in Hollywood.

Source: http://www.nbcnews.com/entertainment/gandolfini-known-tv-mobster-remembered-fighter-veterans-6C10394822

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Weiner calls rival over voter's gay slur

Christine Quinn said she received a message from Anthony Weiner on Thursday. (Andrew Burton/Getty Images)Former Rep. Anthony Weiner called his New York City mayoral rival Christine Quinn on Thursday to clarify a recent discussion he had with a voter who reportedly used a homophobic slur to describe Quinn.

Quinn, who is gay, told reporters on Friday she received a phone message from Weiner on Thursday after he came under fire for not strongly admonishing a voter he met during a campaign event who reportedly referred to Quinn as a ?dyke.?

The interaction, detailed in a Washington Post story, said Weiner did not scold the woman until after he noticed a reporter?s ?incredulous reaction." Weiner then reportedly told the voter, who apologized, ?It?s OK. It?s not your fault.?

On Thursday, Weiner told reporters that he recalled admonishing the woman but insisted he did not recall any further interaction. He reaffirmed his support for gay rights and said he would not tolerate ?any utterance of any type of slur against any community.?

On Friday, Quinn said she was ?grateful? that Weiner clarified the interaction in his phone message to her?but she stopped short of saying whether he explicitly apologized.

?It is incredibly important for all New Yorkers?but particularly those in public life?to make very clear that in this city, the most diverse city in the world, in the city where the LGBT civil rights movement was born, that that type of language cannot be tolerated,? Quinn said, according to Politicker. ?I think all of us need to re-commit to making sure that whenever we hear language of any type that is demeaning, derogatory, racists, sexist, homophobic, anything of that nature, that we speak out against it.?

Weiner issued a stronger apology in a statement issued on Twitter by his spokeswoman Barbara Morgan, insisting again that he did not believe the woman?s comment was ?appropriate.?

?If the impression is that I did, I apologize because behavior like this will absolutely not be tolerated in my administration,? Weiner said.

Source: http://news.yahoo.com/blogs/ticket/weiner-called-quinn-clarify-talk-voter-used-gay-175640568.html

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Facebook announces Video on Instagram to take on Vine

Facebook announces Video on Instagram to take on Vine

With recent moves to add hashtag support, verified Pages, comments with inline photo embeds and more, it appears that Facebook is ready to take on competing social networks. It should come as no surprise to us, then, that it's putting its acquisition of Instagram to good use by introducing a service -- aptly called Video on Instagram --that rivals Vine, a similar service now owned by Twitter.

Instagram's version will be accessed by an icon on the bottom right corner of the app, and you'll be able to record up to 15 seconds of video, using your choice of 13 new filters exclusively for the service. Contrary to its major competitor, this particular service (which will be available on iOS and Android versions from day one, and can be viewed on the web as well) won't loop the video on an endless basis -- rather, you'll see it pop up in your feed and the video will run just once. In addition to filters, Instagram has introduced a stabilization feature called Cinema. Instagram's blog post and video showing the new service can be found after the break, and the iOS version is already live on the App Store. Sadly, Instagram had no news about when we can expect to see the app on Windows Phone, but the team has been "talking with [Microsoft] and learning." And folks, please promise you won't go crazy on the cat videos.

Update: Both iOS and Android apps are now available in their respective stores.

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Source: http://www.engadget.com/2013/06/20/facebook-video-instagram/?utm_medium=feed&utm_source=Feed_Classic&utm_campaign=Engadget

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One man's plan to save humanity in a Kansas mine

ATCHISON, Kan. (AP) ? After most of the world's population is wiped off the map by a wayward meteorite or hail of nuclear missiles, the survival of the human race might just depend on a few thousand people huddled in recreational vehicles deep in the bowels of an eastern Kansas mine.

That's the vision of a California man who is creating what he calls the world's largest private underground survivor shelter, using a complex of limestone caves dug more than 100 years ago beneath gently rolling hills overlooking the Missouri River.

"I do believe I am on a mission and doing a spiritual thing," said Robert Vicino, who has purchased a large portion of the former U.S. Army storage facility on the southeast edge of Atchison, about 50 miles northwest of Kansas City, Mo. "We will certainly be part of the genesis."

Before it comes time to ride out Armageddon or a deadly global pandemic, though, Vicino says the Vivos Survival Shelter and Resort will be a fun place for members to take vacations and learn assorted survival skills to prepare them for whatever world-changing catastrophe awaits.

Jacque Pregont, president of the Atchison Chamber of Commerce, said some people think the shelter plan sounds creepy or that Vicino has "lost his mind," while others are excited because they will finally get a chance to tour the property.

Atchison is known as the birthplace of Amelia Earhart and one of the most haunted towns in Kansas, Pregont said, so the survival shelter is likely to add to the town's tourism draw.

"It's quirky, and quirky gets attention," she said.

Recent Hollywood movies have done big business exploring themes about threats to the human race, either through climate shifts, meteor impacts or zombie invasions. And the National Geographic Channel show, "Doomsday Preppers," documents the efforts of Americans who are preparing for the end of the world with elaborate shelters and plenty of freeze-dried rations.

Paul Seyfried, who belongs to a group that promotes preparing for manmade or natural disasters, said Americans have become complacent ever since the death of John F. Kennedy, the last president who urged people to build fallout shelters.

"There has been no war on our soil in over 100 years, so the horror of war is not stamped indelibly in Americans' minds," said Seyfried, a member of The American Civil Defense Association's advisory board.

Ken Rose, a history professor at California State University-Chico, is an outspoken critic of underground shelters. Though he acknowledged that interest in underground shelters is growing, he called projects like the Kansas facility a "colossal waste of time and money."

"Some people are just obsessed by this idea," Rose said. "... Without minimizing the terror threat here today, the threats were much greater at the height of the Cold War. At least then anxiety was based on a realistic scenario."

The Kansas caverns are 100 feet to 150 feet below the surface and have a constant natural temperature in the low 70s. They are supported by thick limestone pillars six times stronger than concrete and will have blast doors built to withstand a one-megaton nuclear explosion as close as 10 miles away, Vicino said.

Other than being surrounded by more than a mile and a half of 6-foot-high chain-link fence topped with sharp rows of barbed wire, the land above ground isn't distinguishable from expanses of hills and trees that surround it. The proposed shelter's entrances ? nondescript concrete loading docks tucked discretely into the wooded hillside ? are easily defensible against any potential intruders provided there's not a full-scale military attack, Vicino said.

The Army used the caverns ? created by limestone mining operations that started in the late 1880s ? for decades as a storage facility before putting them up for auction last year. The winning bid in December was $1.7 million, but financing fell through and the site was put up for sale again.

Springfield, Mo., investor Coby Cullins submitted his winning $510,000 bid for the property in early April, and he immediately started looking for ways to use it. One of his ideas was to lease the land to a company that builds survival bunkers.

Vicino, whose company is based in Del Mar, Calif., said he received an email from Cullins and flew to Kansas two days later to check out the property. Vicino agreed to purchase 75 percent of the complex, rather than lease it, while Cullins retained the rest and is marketing it to local businesses.

The complex consists of two fully lighted, temperature-controlled mines with concrete floors. The east cave, which Cullins owns, encompasses about 15 acres and contains offices, vaults, restrooms and other developed work spaces. The much larger west cave, which covers about 45 acres, is mostly undeveloped and will be converted into the Vivos facility.

The shelter will have enough space for more than 1,000 RVs and up to about 5,000 people. Members will be charged $1,000 for every lineal foot of their RV to purchase their space, plus $1,500 per person for food. That means a person who plans to park a 30-foot vehicle in the shelter with four people inside will pay $30,000 for the space and $6,000 for food.

Actual sales won't begin until a "critical mass" of reservations are received and processed, Vicino said, which hasn't happened yet at the Kansas shelter.

Vivos also owns a shelter in Indiana with room for 80 people to live comfortably for up to a year. There, members pay $50,000 per adult and $35,000 per child, so a family with two adults and two children would have to come up with $170,000 to be part of the post-apocalyptic generation.

Purchasers will be required to pay for the full balance before taking possession of their shelter space, though the company has offered limited financing in the past with a sizable down payment.

Vicino says he won't say specifically where the Indiana shelter or any of his smaller facilities are located because he fears there would be anarchy in the event of a world-changing catastrophe.

And it doesn't matter who comes knocking at the "moment of truth," Vicino said, they're probably not getting in.

"I've heard people say, 'I will just show up at the door,'" he said. "Our response is, 'great, where is the door?' At our secret shelters, you don't know where to go, and your cash will be worthless at that time."

Source: http://news.yahoo.com/developer-kan-caverns-could-preserve-human-race-071249444.html

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G-8 NOTEBOOK: Cameron Tweets WWII bomb at G-8 site

ENNISKILLEN, Northern Ireland (AP) -- Well, that was close. Or was it? Shortly after wrapping up one of the most peaceful Group of Eight summits in recent memory, Prime Minister David Cameron let a cat out of the bag.

"Now #G8UK is over I can reveal a bomb was found in Lough Erne," he posted on Twitter. "It was American from WW2. Apparently they had a practice mortar range here!"

Not to worry, however. Northern Ireland police mounted a massive security operation to protect the Loch Erne resort west of Enniskillen involving thousands of officers, restricted airspace and contingencies for protesters. While the security appeared aimed at more modern threats, it clearly paid off.

Police in Northern Ireland confirmed that "during ongoing checks" ahead of the G-8, a suspicious item was discovered on June 12, which later was confirmed to be military ordnance from WWII.

___

While Dad was off deep in talks of pressing world affairs, presidential daughters Malia and Sasha Obama joined their mom for lunch with Irish rock star Bono.

Hundreds of onlookers had gathered by the time Bono and wife Ali Hewson arrived at the U2 frontman's favored local pub, Finnegan's, in the exclusive Dublin suburb of Dalkey. "I thought this was supposed to be secret," the singer said to the crowd. The spectators may have been tipped off by the U.S. flag flying outside and the restaurant closed to the public amid tight security.

The Obamas dined on fish and chips, natch, with cookies and tea for dessert. "We talked about everything and nothing," said Bono, who is well known to world leaders as an anti-poverty campaigner. "It was a family lunch."

Earlier Tuesday, the Obama ladies had a private guided tour of the Wicklow Mountains National Park south of Dublin. The sun was shining, but the heat brought swarms of unwelcome visitors ? swarming flies called "midges" that forced 12-year-old Sasha to flail her arms as she toured monastic ruins.

But the flies didn't dampen her spirit. Sasha closed the door to the ruins of a 1,500-year-old church, then turned to 14-year-old Malia and the first lady for a high-five.

___

Cameron has been pushing three T's throughout the G-8 summit in Northern Ireland ? tax, trade and transparency.

He might've tacked on one more as he closed out the gathering of world leaders, making a hard sell for tourism to rejuvenate Northern Ireland's economy.

Cameron acknowledged that many people had a negative impression of Northern Ireland after the country's divisions and struggles. He said that he'd had many goals for the summit ? and improving the image of Northern Ireland was one of them through a bit of free advertising.

You couldn't put a value on the "infomercial" that was the G-8 summit, he told reporters. He boasted that all his fellow G-8 leaders were impressed by their meeting site.

"It's only when you come and see it for yourself that you just see what a magnificent part of the United Kingdom this is," he said.

Cameron isn't the only one hoping that the free advertising pays off. The Loch Erne resort where he spoke went bankrupt in 2011.

___

While Russian President Vladimir Putin might be more prone to pose for sporting pictures, it appears Cameron might have one-upped him ? if anyone can find the evidence.

Cameron told reporters he'd gone for a dip in Loch Erne, saying a swim in the chilly waters was a good way to prepare for a day of tough negotiations.

"It certainly wakes you up in the morning and gets you going for a hard day of chairing these meetings," he told a press conference.

The prime minister's spokesman described the experience as "brisk" in earlier remarks to reporters. Temperatures around Loch Erne were in the low 60s ? but felt much chillier.

Source: http://news.yahoo.com/g-8-notebook-cameron-tweets-115704250.html

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মঙ্গলবার, ২১ মে, ২০১৩

Breakup of physician, drug company relationship could improve health care, cut cost

May 20, 2013 ? A new report suggests that improved health care and significant reductions in drug costs might be attained by breaking up the age-old relationship between physicians and drug company representatives who promote the newest, more costly and often unnecessary prescription drugs.

This system, which has been in place for decades, at one time benefited doctors by keeping them up to date on new medications, and always provided generous amounts of "free" samples to get patients started on the newest drugs, as well as other supplies and gifts.

But it's actually a powerful marketing process into which the pharmaceutical industry pours tens of billions of dollars a year, with more than 90,000 drug representatives providing gifts and advice. There is one drug representative for every eight doctors in the United States. This doesn't necessarily serve the best interests of the patient in terms of economy, efficacy, safety or accuracy of information, experts say.

In one of the first reports of its type -- titled "Breaking Up is Hard to Do" -- researchers from Oregon State University, Oregon Health & Science University and the University of Washington outlined the deliberate process that one central Oregon medical clinic went through to remove drug company representatives from their practice. It explored the obstacles they faced and the ultimate, successful result. The findings were just published in the Journal of the American Board of Family Medicine.

The study found that avoiding conflicts of interest and becoming "pharma-free" is possible, but not easy.

"This is a culture change, one that's already happening but still has a ways to go, especially in smaller private practices," said Dr. David Evans, now with the Department of Family Medicine at the University of Washington, and previously a physician at the Madras, Ore., clinic featured in the article.

"The relationship between physicians and drug company representatives goes back generations, and it took a methodical, deliberate campaign to change it," Evans said. "We ultimately decided something had to be done when our medical clinic was visited by drug reps 199 times in six months. That number was just staggering."

Part of what allows the change, the researchers said, is that information on new medications is now available in many other forums. These may have less bias and be more evidence-based than the material traditionally provided by the pharmaceutical industry, which wanted to sell the latest product. In the Madras clinic, the physicians replaced information previously supplied by drug reps with monthly meetings to stay current on new medications, based on peer-reviewed, rather than promotional literature.

"In the past 5-10 years there's been more of a move toward what we call 'academic detailing,' in which universities and other impartial sources of information can provide accurate information without bias," said Daniel Hartung, assistant professor in the OSU College of Pharmacy. "This is being supported by some states and the federal government, and it's a move in the right direction."

Moves to separate the drug industry from the practice of medicine have been more aggressive in large medical teaching hospitals, Hartung said, but much less so in smaller private practice. Of the 800,000 physicians in the U.S., only 22 percent practice in academic settings, the study noted, and 84 percent of primary care physicians still have close relationships with the pharmaceutical industry.

The stakes can be high, the researchers said. In the study example, the "sample cabinet" of medications at the Madras clinic, provided for free by the pharmaceutical representatives, had an average price of $90 for a month's supply of the medications. Less expensive, generic medications were identified for 38 of the 46 sample drugs, which would have cost $22 a month.

The new analysis explored the necessary steps that a private clinic can take to help address this concern, including quantifying the clinic-industry relationship, anticipating clinician and staff concerns, finding new ways to provide up-to-date information, and educating patients and the public.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/~3/qnl5Jy21UwU/130520133749.htm

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Crazy ants vs. Fire ants: Who's winning? (+video)

Crazy ants are taking over areas once occupied by fire ants in the South. The sting of Crazy ants isn't as painful and migrate slowly. But Crazy ants multiply faster.

By Douglas Main,?LiveScience / May 18, 2013

Invasive fire ants have been a thorn in the sides of Southerners for years. But another invasive species, the so-called "crazy" ant ? that many describe as being worse ? has arrived and is displacing fire ants in several places.

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"When you talk to folks who live in the invaded areas, they tell you they want their fire ants back," said Edward LeBrun, a researcher at the University of Texas at Austin, in a statement from the school. "Fire ants are in many ways very polite. They live in your yard. They form mounds and stay there, and they only interact with you if you step on their mound."

Crazy ants, on the other hand, "go everywhere," invading homes and nesting in walls and crawlspaces, even damaging electrical equipment by swarming inside appliances. [Image Gallery: Ants of the World]

A study published in the April issue of the journal Biological Invasions found that in areas infested with crazy ants, few to no fire ants were present. Exactly how they are able to outcompete fire ants is so far unknown. In areas with crazy ants, the researchers also found greatly diminished numbers of native ant species, according to the study.

Fire ants are known for their painful stings and have spread through the Southeast since arriving from South America in the 1930s. Crazy ants were first discovered in Houston in 2002, and they have already spread to coastal areas from Texas to Florida, according to the researchers. Although the "crazies" don't have as painful a sting as fire ants, they multiply in even greater numbers. They are also difficult to control since they don't eat the same poison baits as fire ants do, the statement noted.

Last year, the crazy ant species was identified as Nylanderia fulva, which hails from northern Argentina and southern Brazil, according to a 2012 study in PLOS ONE. It's also known as the tawny crazy ant and was previously named the Rasberry crazy ant after the exterminator Tom Rasberry, who first discovered it. The "crazy" moniker comes from the ant's quick, seemingly random movements.

Luckily, the crazy ant doesn't spread as quickly as the fire ant, advancing only 650 feet (200 meters) per year on its own, the release noted. Therefore, it's vital that people don't accidentally transport the ant, the prime method by which it has spread, according to the release.

Email?Douglas Main?or follow him on?Twitter?or?Google+. Follow us @livescience, ?Facebook?or ?Google+. Article originally on LiveScience.com.

Copyright 2013 LiveScience, a TechMediaNetwork company. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.

Source: http://rss.csmonitor.com/~r/feeds/csm/~3/3FwH4s3kfNU/Crazy-ants-vs.-Fire-ants-Who-s-winning-video

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Computational tool translates complex data into simplified 2-dimensional images

Computational tool translates complex data into simplified 2-dimensional images [ Back to EurekAlert! ] Public release date: 19-May-2013
[ | E-mail | Share Share ]

Contact: Karin Eskenazi
ket2116@columbia.edu
212-342-0508
Columbia University Medical Center

May allow doctors to track progression of cancer, response to treatment, and risk of relapse

NEW YORK, NY (May 19, 2013) In their quest to learn more about the variability of cells between and within tissues, biomedical scientists have devised tools capable of simultaneously measuring dozens of characteristics of individual cells. These technologies have led to new challenges, however, as scientists now struggle with how to make sense of the resulting trove of data. Now a solution may be at hand. Researchers at Columbia University and Stanford University have developed a computational method that enables scientists to visualize and interpret "high-dimensional" data produced by single-cell measurement technologies such as mass cytometry. The method, published today in the online edition of Nature Biotechnology, has particular relevance to cancer research and therapeutics.

Researchers now understand that cancer within an individual can harbor subpopulations of cells with different molecular characteristics. Groups of cells may behave differently from one another, including in how they respond to treatment. The ability to study single cells, as well as to identify and characterize subpopulations of cancerous cells within an individual, could lead to more precise methods of diagnosis and treatment.

"Our method not only will allow scientists to explore the heterogeneity of cancer cells and to characterize drug-resistant cancer cells, but also will allow physicians to track tumor progression, identify drug-resistant cancer cells, and detect minute quantities of cancer cells that increase the risk of relapse," said co-senior author Dana Pe'er, PhD, associate professor of biological sciences and systems biology at Columbia. The other co-senior author is Garry P. Nolan, PhD, professor of microbiology & immunology at Stanford.

The method, called viSNE (visual interactive Stochastic Neighbor Embedding), is based on a sophisticated algorithm that translates high-dimensional data (e.g., a dataset that includes many different simultaneous measurements from single cells) into visual representations similar to two-dimensional "scatter plots"the simple graphs with X and Y axes that many people first encounter in high school math and biology. "Basically, viSNE provides a way to visualize very high-dimensional data in two dimensions, while maintaining the most important organization and structure of the data," said Dr. Pe'er. "Color is used as a third dimension to enable users to interactively visualize various features of the cells."

The viSNE software can analyze measurements of dozens of molecular markers. In the two-dimensional maps that result, the distance between points represents the degree of similarity between single cells. The maps can reveal clearly defined groups of cells with distinct behaviors (e.g., drug resistance) even if they are only a tiny fraction of the total population. This should enable the design of ways to physically isolate and study these cell subpopulations in the laboratory.

Although the algorithm underlying the method is complex, Dr. Pe'er expects that all researchers, no matter their level of mathematical expertise, will be able to use viSNE.

To demonstrate the software's utility, Dr. Pe'er and her colleagues used mass cytometry and viSNE to study bone marrow cells from patients with acute myeloid leukemia. Currently, clinicians can incorporate at most 4 to 8 markers to assess the cells. Because mass cytometry and viSNE can incorporate many more markers, viSNE is able to identify more subtle differences between cells. Using the algorithm, Dr. Pe'er and her colleagues were able to reveal previously unrecognized heterogeneity in the bone marrow cells they studied.

The researchers also showed that viSNE could detect minimal residual disease (MRD) extremely small quantities of cancer cells that persist after chemotherapy and raise the risk of recurrence. "In blinded tests, we were able to find as few as 20 cancer cells out of tens of thousands of healthy cells," said Dr. Pe'er. Such a small quantity of cells is extremely difficult to detect, even by the most experienced pathologist.

"The ability to detect MRD is critical for curing cancer," added Dr. Pe'er. "Eliminating even 99.9 percent of a tumor doesn't bring about a cure. You have to be able to find, and then eliminate, the tiny populations of cells that can survive therapy and lead to disease relapse."

###

The paper is titled, "viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia." The other authors are: El-ad David Amir (Columbia), Kara L. Davis (Stanford University), Michelle D. Tadmor (Columbia), Erin F. Simonds (Stanford), Jacob H. Levine (Columbia), Sean C. Bendall (Stanford), Daniel K. Shenfeld (Columbia), and Smita Krishnaswamy (Columbia).

The study was supported by grants from the National Science Foundation (MCB-1149728), National Institutes of Health Roadmap Initiative, the NIH Director's New Innovator Award Program (1-DP2-OD002414-01), and the National Centers for Biomedical Computing (1U54CA121852-01A1).

The authors declare no financial or other conflicts of interest.

Columbia University Medical Center provides international leadership in basic, preclinical, and clinical research; medical and health sciences education; and patient care. The medical center trains future leaders and includes the dedicated work of many physicians, scientists, public health professionals, dentists, and nurses at the College of Physicians and Surgeons, the Mailman School of Public Health, the College of Dental Medicine, the School of Nursing, the biomedical departments of the Graduate School of Arts and Sciences, and allied research centers and institutions. Columbia University Medical Center is home to the largest medical research enterprise in New York City and State and one of the largest faculty medical practices in the Northeast. For more information, visit cumc.columbia.edu or columbiadoctors.org.


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AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.


Computational tool translates complex data into simplified 2-dimensional images [ Back to EurekAlert! ] Public release date: 19-May-2013
[ | E-mail | Share Share ]

Contact: Karin Eskenazi
ket2116@columbia.edu
212-342-0508
Columbia University Medical Center

May allow doctors to track progression of cancer, response to treatment, and risk of relapse

NEW YORK, NY (May 19, 2013) In their quest to learn more about the variability of cells between and within tissues, biomedical scientists have devised tools capable of simultaneously measuring dozens of characteristics of individual cells. These technologies have led to new challenges, however, as scientists now struggle with how to make sense of the resulting trove of data. Now a solution may be at hand. Researchers at Columbia University and Stanford University have developed a computational method that enables scientists to visualize and interpret "high-dimensional" data produced by single-cell measurement technologies such as mass cytometry. The method, published today in the online edition of Nature Biotechnology, has particular relevance to cancer research and therapeutics.

Researchers now understand that cancer within an individual can harbor subpopulations of cells with different molecular characteristics. Groups of cells may behave differently from one another, including in how they respond to treatment. The ability to study single cells, as well as to identify and characterize subpopulations of cancerous cells within an individual, could lead to more precise methods of diagnosis and treatment.

"Our method not only will allow scientists to explore the heterogeneity of cancer cells and to characterize drug-resistant cancer cells, but also will allow physicians to track tumor progression, identify drug-resistant cancer cells, and detect minute quantities of cancer cells that increase the risk of relapse," said co-senior author Dana Pe'er, PhD, associate professor of biological sciences and systems biology at Columbia. The other co-senior author is Garry P. Nolan, PhD, professor of microbiology & immunology at Stanford.

The method, called viSNE (visual interactive Stochastic Neighbor Embedding), is based on a sophisticated algorithm that translates high-dimensional data (e.g., a dataset that includes many different simultaneous measurements from single cells) into visual representations similar to two-dimensional "scatter plots"the simple graphs with X and Y axes that many people first encounter in high school math and biology. "Basically, viSNE provides a way to visualize very high-dimensional data in two dimensions, while maintaining the most important organization and structure of the data," said Dr. Pe'er. "Color is used as a third dimension to enable users to interactively visualize various features of the cells."

The viSNE software can analyze measurements of dozens of molecular markers. In the two-dimensional maps that result, the distance between points represents the degree of similarity between single cells. The maps can reveal clearly defined groups of cells with distinct behaviors (e.g., drug resistance) even if they are only a tiny fraction of the total population. This should enable the design of ways to physically isolate and study these cell subpopulations in the laboratory.

Although the algorithm underlying the method is complex, Dr. Pe'er expects that all researchers, no matter their level of mathematical expertise, will be able to use viSNE.

To demonstrate the software's utility, Dr. Pe'er and her colleagues used mass cytometry and viSNE to study bone marrow cells from patients with acute myeloid leukemia. Currently, clinicians can incorporate at most 4 to 8 markers to assess the cells. Because mass cytometry and viSNE can incorporate many more markers, viSNE is able to identify more subtle differences between cells. Using the algorithm, Dr. Pe'er and her colleagues were able to reveal previously unrecognized heterogeneity in the bone marrow cells they studied.

The researchers also showed that viSNE could detect minimal residual disease (MRD) extremely small quantities of cancer cells that persist after chemotherapy and raise the risk of recurrence. "In blinded tests, we were able to find as few as 20 cancer cells out of tens of thousands of healthy cells," said Dr. Pe'er. Such a small quantity of cells is extremely difficult to detect, even by the most experienced pathologist.

"The ability to detect MRD is critical for curing cancer," added Dr. Pe'er. "Eliminating even 99.9 percent of a tumor doesn't bring about a cure. You have to be able to find, and then eliminate, the tiny populations of cells that can survive therapy and lead to disease relapse."

###

The paper is titled, "viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia." The other authors are: El-ad David Amir (Columbia), Kara L. Davis (Stanford University), Michelle D. Tadmor (Columbia), Erin F. Simonds (Stanford), Jacob H. Levine (Columbia), Sean C. Bendall (Stanford), Daniel K. Shenfeld (Columbia), and Smita Krishnaswamy (Columbia).

The study was supported by grants from the National Science Foundation (MCB-1149728), National Institutes of Health Roadmap Initiative, the NIH Director's New Innovator Award Program (1-DP2-OD002414-01), and the National Centers for Biomedical Computing (1U54CA121852-01A1).

The authors declare no financial or other conflicts of interest.

Columbia University Medical Center provides international leadership in basic, preclinical, and clinical research; medical and health sciences education; and patient care. The medical center trains future leaders and includes the dedicated work of many physicians, scientists, public health professionals, dentists, and nurses at the College of Physicians and Surgeons, the Mailman School of Public Health, the College of Dental Medicine, the School of Nursing, the biomedical departments of the Graduate School of Arts and Sciences, and allied research centers and institutions. Columbia University Medical Center is home to the largest medical research enterprise in New York City and State and one of the largest faculty medical practices in the Northeast. For more information, visit cumc.columbia.edu or columbiadoctors.org.


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Source: http://www.eurekalert.org/pub_releases/2013-05/cumc-ctt051613.php

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